BS EN 16418:2014

This standard BS EN 16418:2014 Paper and board. Determination of the cytotoxicity of aqueous extracts using a metabolically competent hepatoma cell line (HepG2) is classified in these ICS categories:

• 85.060 Paper and board

This European Standard specifies a test method for the laboratory assessment of the potential cytotoxic effect of paper and board intended to come into contact with foodstuffs using specifically the HepG2 cell line.

Compared to the EN 15845^[1], HepG2 cells are more representative of a human oral exposure to xenobiotics, due to the presence in the cells of phase I, II and III enzymes of the metabolism.