BS EN 17250:2020

This standard BS EN 17250:2020 Foodstuffs. Determination of ochratoxin A in spices, liquorice, cocoa and cocoa products by IAC clean-up and HPLC-FLD is classified in these ICS categories:

• 67.220.10 Spices and condiments
• 67.140.30 Cocoa

This document specifies a procedure for the determination of ochratoxin A (OTA) in chilli, paprika, black and white pepper, nutmeg, spice mix, liquorice (root and extracts), cocoa and cocoa products by high performance liquid chromatography (HPLC) with immunoaffinity column clean-up and fluorescence detection (FLD).

This method has been validated in interlaboratory studies via the analysis of both naturally contaminated and spiked samples ranging from 1,0 ?g/kg to 84,9 ?g/kg for spices (paprika and chili [5], black and white pepper, nutmeg and spice mix [6]), ranging from 7,7 ?g/kg to 96,8 ?g/kg for liquorice and liquorice products [7] and ranging from 2,1 ?g/kg to 26,3 ?g/kg for cocoa and cocoa products [6].

For further information on the validation, see Clause 10 and Annex B.