ČSN EN ISO 19040-2

This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with a genetically modified yeast strain Arxula adeninivorans. This reporter gene assay is based on the activation of the human estrogen receptor alpha. Arxula adeninivorans is a highly robust and salt- and temperature-tolerant test organism and is especially suitable for the analysis of samples with high salinity (conductivity up to 70 mS/cm). The test organism can be cultivated in medium with sodium chloride content up to 20 %. This method is applicable to: fresh water; waste water; sea water; brackish water; aqueous extracts and leachates; eluates of sediments (fresh water); pore water; aqueous solutions of single substances or of chemical mixtures; drinking water.