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sklademVydáno: 2008-03-31
DD ISO/TS 23893-2:2007
Water quality. Biochemical and physiological measurements on fish Determination of ethoxyresorufin-O-deethylase (EROD)
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| Označení normy: | DD ISO/TS 23893-2:2007 |
| Počet stran: | 26 |
| Vydáno: | 2008-03-31 |
| ISBN: | 978 0 580 53706 6 |
| Status: | Standard |
DESCRIPTION
DD ISO/TS 23893-2:2007
This standard DD ISO/TS 23893-2:2007 Water quality. Biochemical and physiological measurements on fish is classified in these ICS categories:
- 13.060.70 Examination of biological properties of water
This part of ISO 23893 specifies a method for measuring the ethoxyresorufin-O-deethylase (EROD) enzyme activity on a post-mitochondrial fraction of fish liver homogenate (subcellular fraction in which the EROD activity is located) employing a cell or microplate fluorimetric method.
It applies to fish that are sampled in their natural environment (fresh water or salt water) or exposed to substances or effluents in a laboratory.
This method is applicable for EROD values greater than or equal to 1 pmol/(min·mg) of proteins. A higher sensitivity may be achieved by using a cell (test tube) procedure.