PD ISO/TS 21569-2:2021
Molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products Construct-specific real-time PCR method for detection of event FP967 in linseed and linseed products
| Označení normy: | PD ISO/TS 21569-2:2021 |
| Počet stran: | 18 |
| Vydáno: | 2021-08-04 |
| ISBN: | 978 0 539 15975 2 |
| Status: | Standard |
PD ISO/TS 21569-2:2021
This standard PD ISO/TS 21569-2:2021 Molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products is classified in these ICS categories:
- 67.050 General methods of tests and analysis for food products
This document specifies a procedure for the detection of a DNA sequence present in a genetically modified linseed (Linum usitatissimum) line (event FP967, also named as “CDC Triffid”). For this purpose, extracted DNA is used in a real-time PCR and the genetic modification (GM) is specifically detected by amplification of a 105 bp DNA sequence representing the transition between the nopaline synthase gene terminator (Tnos) from Agrobacterium tumefaciens and the dihydrofolate reductase gene (dfrA1) from a Class 1 integron of Escherichia coli.
The method described is applicable for the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix for the purpose of analysis.